Erbium:YAG Laser and Cultured Epidermis in the Surgical Therapy of Stable Vitiligo

• DOI: 10.1001/archderm.139.10.1303
• Publication Date: 2003-10-20T20:31:53Z
• Authors: Liliana Guerra; Grazia Primavera; Desanka Raskovic; Graziella Pellegrini; Osvaldo Golisano; Sergio Bondanza; Patrizia Paterna; Giulio Sonego; Tommaso Gobello; Francesco Atzori; Paolo Piazza; Antonio Luci; Michele De Luca
• Keywords: Adult; Male; Time Factors; Adolescent; Vitiligo; Middle Aged; Transplantation, Autologous; 3. Good health; 03 medical and health sciences; Treatment Outcome; 0302 clinical medicine; Humans; Female; Laser Therapy; Epidermis; Cells, Cultured; Stem cell; Epithelial cell; Regenerative Medicine; Follow-Up Studies

To induce complete and reproducible repigmentation of large "stable" vitiligo lesions by means of autologous cultured epidermal grafts using a rapid, simple, and minimally invasive surgical procedure.Achromic epidermis was removed by means of appropriately settled erbium:YAG laser, and autologous epidermal grafts were applied onto the recipient bed. Melanocyte content was evaluated by dopa reaction. The percentage of repigmentation was calculated using a semiautomatic image analysis system.A biosafety level 3-type cell culture facility, a surgical ambulatory department, and a dermatological department in a hospital.Twenty-one patients with different types of vitiligo were admitted to the study and treated with autologous cultured epidermal grafts. Inclusion criteria were failure of at least 2 standard medical approaches; no therapy for at least 12 months; no progression of old lesions or appearance of new lesions; no Koebner phenomenon within the past 18 months; and no autoimmune disorders.The average percentage of repigmentation in 21 patients was 75.9% (1759.7 cm2 repigmented/2315.8 cm2 transplanted). Three patients showed a reactivation of their vitiligo and did not show repigmentation. The remaining 18 patients, with 43 distinct lesions, showed an average percentage of repigmentation of 90% (1759.7 cm2 repigmented/1953.4 cm2 transplanted).Under appropriate conditions, cultured epidermal grafts induce complete repigmentation of stable vitiligo lesions. Erbium:YAG laser surgery can supply a fast and precise tool for disepithelialization, hence allowing treatment of large vitiligo lesions during a single surgical operation.