Culturing, Storage, and Quantification of Rotaviruses

• DOI: 10.1002/9780471729259.mc15c03s15
• Publication Date: 2009-11-05T07:21:12Z
• Authors: Michelle Arnold; John T. Patton; Sarah M. McDonald
• Keywords: Isolation; African Green Monkey

Abstract Group A rotaviruses (RVs) infect the young of numerous animal species and cause acute gastroenteritis. Cultivation human RVs in cells requires proteolytic activation viral attachment protein using trypsin. Continuous cell lines, such as rhesus monkey kidney cells, well primary are routinely used for growth characterization RVs. Isolation cultivation from clinical fecal specimens is difficult adaptation to vitro multiple rounds passage cells. Following growth, RV stocks can be purified by centrifugation, if required, quantified plaque assay or fluorescence focus assay. This unit describes easily applicable procedures culturing, storage, quantification Curr. Protoc. Microbiol . 15:15C.3.1‐15C.3.24. © 2009 John Wiley & Sons, Inc.