Microscaffolds by Direct Laser Writing for Neurite Guidance Leading to Tailor‐Made Neuronal Networks

• DOI: 10.1002/adbi.201800329
• Publication Date: 2019-03-07T11:14:29Z
• Authors: Cornelius Fendler; Christian Denker; Jann Harberts; Parisa Bayat; Robert Zierold; Gabriele Loers; Markus Münzenberg; Robert H. Blick
• Keywords: Neurite; Microscale chemistry; Biological neural network

Abstract While modern day integrated electronic circuits are essentially designed in a 2D fashion, the brain can be regarded as 3D circuit. The thus enhanced connectivity enables much more complex signal processing compared to conventional circuits. Recent technological advances development of nano/microscale structuring have led artificial neuron culturing platforms, which surpass possibilities classical cultures. In this work, vitro neuronal networks is demonstrated by determining predefined pathways through topological and chemical neurite guiding. Tailor‐made substrates microtowers freestanding microtubes fabricated using direct laser writing two‐photon polymerization. first scaffold design that allows for site‐specific cell attachment directed outgrowth single neurites along defined paths arranged freely all dimensions, build with low density, presented. neurons cultured scaffolds show characteristic electrophysiological properties vital cells after 10 d vitro. introduced offers promising concept future network studies on tailor‐made specific connections beyond 2D.