Molecular Sieving on the Surface of a Protein Provides Protection Without Loss of Activity

• DOI: 10.1002/adfm.201202227
• Publication Date: 2012-11-15T22:26:52Z
• Authors: Mi Liu; Pasquale Tirino; Milos Radivojevic; Daniel J. Phillips; Matthew I. Gibson; Jean‐Christophe L...; Marc A. Gauthier
• Keywords: PEGylation; Biomolecule

Abstract Tethering polymers to surfaces represents the cornerstone of a wide range applications, including stabilization colloids/biomolecules and preparation functional coatings. Unfortunately, despite prevalence protein‐tethered in pharmaceutical sector, analysis such polymer monolayers on molecular level is difficult. In this work, simple 1 H NMR spectroscopy catalytic properties α‐chymotrypsin are used analyze conformational/permeability protein‐bound poly(oligoethyleneglycol monomethylether methacrylate) (pOEGMA), biocompatible comb‐polymer interest biomedical field. By analyzing >100 distinct conjugates pOEGMA, detailed picture behavior pOEGMA surface protein was obtained. Remarkably, control conformation inter‐penetration produced thus far overlooked sieving effect. The application effect for “smart” PEGylation proteins portrayed, from which insight provided design other therapeutic bioconjugates coatings with selective permeability properties.