InVADE: Integrated Vasculature for Assessing Dynamic Events

• DOI: 10.1002/adfm.201703524
• Publication Date: 2017-10-16T11:12:49Z
• Authors: Benjamin Fook Lun...; Locke Davenport H...; Rick Xing Ze Lu; Stasja Drecun; Milica Radisic; Boyang Zhang
• Keywords: Crosstalk

Abstract Drug screening with simplified 2D cell culture and relevant animal testing fail to predict clinical outcomes. With the rising cost of drug development, predictive 3D tissue models human cells are in urgent demand. Establishing vascular perfusion tissues has always been a challenge, but it is necessary mimic transport capture complex interorgan crosstalk. Here, versatile multiwell plate presented empowered by built‐in microfabricated scaffolds that define space support self‐assembly various parenchymal tissues. In this configuration, assembly organ‐specific function metabolically active liver, free‐contracting cardiac muscle, metastatic solid tumor demonstrated, tracking organ using noninvasive analysis techniques. By linking liver series, demonstrated presence crucial correctly reveal efficacy chemotherapeutic drug, Tegafur. Furthermore, complete cancer metastasis cascade across multiple organs, where escaping from can invade distant connected through continuous interface. This combinatory use scaffold onto standard culturing platform offer important insights into mechanics biological events.