Antibody‐Functionalized Porous Silicon Nanoparticles for Vectorization of Hydrophobic Drugs

• DOI: 10.1002/adhm.201200335
• Publication Date: 2012-11-30T14:17:40Z
• Authors: Emilie Secret; Kevin Smith; Valentina Dubljevic; Eli Moore; Peter Macardle; Bahman Delalat; Mary‐Louise Rogers; Terrance G. Johns; Jean‐Olivier Durand; Frédérique Cunin; Nicolas H. Voelcker
• Keywords: Camptothecin; Bioconjugation; Porous Silicon

Abstract We describe the preparation of biodegradable porous silicon nanoparticles (pSiNP) functionalized with cancer cell targeting antibodies and loaded hydrophobic anti‐cancer drug camptothecin. Orientated immobilization antibody on pSiNP is achieved using novel semicarbazide based bioconjugate chemistry. To demonstrate generality this approach, three MLR2, mAb528 Rituximab are used, which target neuroblastoma, glioblastoma B lymphoma cells, respectively. Successful demonstrated by means flow cytometry immunocytochemistry both lines primary cells. Cell viability assays after incubation pSiNPs show selective killing cells expressing receptor corresponding to attached pSiNP.