Covalent Incorporation of Trehalose within Hydrogels for Enhanced Long‐Term Functional Stability and Controlled Release of Biomacromolecules

• DOI: 10.1002/adhm.201500334
• Publication Date: 2015-06-18T16:54:04Z
• Authors: Timothy M. O'Shea; Matthew J. Webber; Alex A. Aimetti; Robert Langer
• Keywords: Male; 0301 basic medicine; Trehalose; Biocompatible Materials; Hydrogels; Hydrogen Bonding; 3. Good health; Mice, Inbred C57BL; Mice; 03 medical and health sciences; Freeze Drying; Drug Stability; Delayed-Action Preparations; Animals

Hydrogels with covalently incorporated trehalose are synthesized using thiol-ene Michael addition. Trehalose hydrogels afford prolonged stabilization and ­controlled release of model enzymes in vitro vivo as well preservation protein stability under heat ­lyophilization stressors. Strong ­ordered hydrogen bonding interactions within represent a possible mechanism for stabilization. As service to our authors readers, this journal provides supporting information supplied by the authors. Such materials peer reviewed may be re-organized online delivery, but not copy-edited or typeset. Technical support issues arising from (other than missing files) should addressed Please note: The publisher is responsible content functionality any Any queries content) directed corresponding author article.