Modeling Metastatic Colonization in a Decellularized Organ Scaffold‐Based Perfusion Bioreactor

• DOI: 10.1002/adhm.202100684
• Publication Date: 2021-11-04T05:01:05Z
• Authors: Maria Rafaeva; Edward R. Horton; Adina R.D. Jensen; Chris D. Madsen; Raphael Reuten; Oliver Willacy; Christian B. Brøc...; Thomas H. Jensen; Kamilla Westarp Z...; Marina Crespo; Dina S. Grønseth; Sebastian R. Nielsen; Manja Idorn; Per thor Straten; Kristoffer Rohrberg; Iben Spanggaard; Martin Højgaard; Ulrik Lassen; Janine T. Erler; Alejandro E. Mayo...
• Keywords: Decellularization; Ex vivo; Regenerative Medicine

Metastatic cancer spread is responsible for most cancer-related deaths. To colonize a new organ, invading cells adapt to, and remodel, the local extracellular matrix (ECM), network of proteins proteoglycans underpinning all tissues, critical regulator homeostasis disease. However, there major lack in tools to study cell behavior within native 3D ECM. Here, an in-house designed bioreactor, where mouse organ ECM scaffolds are perfused populated with that challenged it, presented. Using specialized bioreactor chamber, it possible monitor microscopically (e.g., proliferation, migration) scaffold. Cancer this system recapitulate signaling observed vivo remodel complex Moreover, bioreactors compatible co-culturing types different genetic origin comprising normal tumor microenvironment. This degree experimental flexibility organ-specific context, opens possibilities cell-cell cell-ECM interplay model diseases controllable ex vivo.