Microfluidics‐Enabled Multimaterial Maskless Stereolithographic Bioprinting

• DOI: 10.1002/adma.201800242
• Publication Date: 2018-05-08T06:36:03Z
• Authors: Amir K. Miri; Daniel Nieto; Luis Iglesias; Hossein Goodarzi ...; Sushila Maharjan; Guillermo U. Ruiz...; Parastoo Khoshakh...; Amir Manbachi; Mehmet Remzi Dokmeci; Shaochen Chen; Su Ryon Shin; Yu Shrike Zhang; Ali Khademhosseini
• Keywords: Stereolithography; Biofabrication; 3D bioprinting; Regenerative Medicine

A stereolithography-based bioprinting platform for multimaterial fabrication of heterogeneous hydrogel constructs is presented. Dynamic patterning by a digital micromirror device, synchronized moving stage and microfluidic device containing four on/off pneumatic valves, used to create 3D constructs. The novel capable fast switching between different (cell-loaded) bioinks, achieve layer-by-layer bioprinting. Compared conventional bioprinters, the system provides unique advantage capability at high spatial resolution. To demonstrate capacity this system, variety are generated, including those based on poly(ethylene glycol) diacrylate (PEGDA) gelatin methacryloyl (GelMA). biocompatibility validated introducing cell-laden GelMA into fabricating cellularized pattern PEGDA frame three concentrations GelMA, loaded with vascular endothelial growth factor, further assessed its neovascularization potential in rat model. proposed robust high-fidelity microstructures demand applications tissue engineering, regenerative medicine, biosensing, which otherwise not readily achievable speed stereolithographic biofabrication platforms.