Oxidized Activated Charcoal Nanozymes: Synthesis, and Optimization for In Vitro and In Vivo Bioactivity for Traumatic Brain Injury

• DOI: 10.1002/adma.202211239
• Publication Date: 2023-03-20T16:05:04Z
• Authors: Emily A. McHugh; Anton V. Liopo; Kimberly Mendoza; Claudia S. Robertson; Gang Wu; Zhe Wang; Weiyin Chen; Jacob L. Beckham; Paul J. Derry; Thomas A. Kent; James M. Tour
• Keywords: Nanomaterials

Carbon-based superoxide dismutase (SOD) mimetic nanozymes have recently been employed as promising antioxidant nanotherapeutics due to their distinct properties. The structural features responsible for the efficacy of these nanomaterials antioxidants are, however, poorly understood. Here, process-structure-property-performance properties coconut-derived oxidized activated charcoal (cOAC) nano-SOD mimetics are studied by analyzing how modifications nanomaterial's synthesis impact size, well elemental and electrochemical particles. These then correlated in vitro bioactivity poly(ethylene glycol)-functionalized cOACs (PEG-cOAC). Chemical oxidative treatment methods that afford smaller, more homogeneous cOAC nanoparticles with higher levels quinone functionalization show enhanced protection against damage bEnd.3 murine endothelioma cells. In an vivo rat model mild traumatic brain injury (mTBI) vascular injury, PEG-cOACs restore cerebral perfusion rapidly same extent former nanotube-derived PEG-hydrophilic carbon clusters (PEG-HCCs) a single intravenous injection. findings provide deeper understanding nanozyme syntheses can be tailored improved bioactivity, set stage translation medical applications.