High‐Efficient Microfluidic Single‐Cell Trapping and Arraying with Absolute Sequential Capture and High Success Rate of Perfect Capture

• DOI: 10.1002/admt.202401018
• Publication Date: 2024-10-18T02:21:23Z
• Authors: Tingting Xuanyuan; Meilin Sun; Jinwei Zhang; Xufang Liu; Danyang Yu; Zeping Liu; Wenming Liu
• Keywords: Absolute (philosophy)

Abstract Methodological improvement to single‐cell manipulation is critical for exploring the fundamentals of cellular life and unraveling biological complexity. Although micro‐manipulation technologies capable precise cell localization have been widely established, scaling existing platforms highly efficient immobilization without sacrificing viability sample quantity has proven challenging. Here, a trapping arraying approach introduced by advancing performance microfluidic mechanical chip. The chip can achieve representative capture with over 99% efficiency at least 75% success rate perfect capture, precisely controlled array, absolute sequential captures loss, maintenance high during whole process. This enables diverse trapping, large‐scale manipulations, dynamic molecular analysis, offers path toward development high‐performance systems.