3D Cellular Architecture Affects MicroRNA and Protein Cargo of Extracellular Vesicles

• DOI: 10.1002/advs.201800948
• Publication Date: 2018-12-20T10:45:44Z
• Authors: Sara Rocha; Joana Carvalho; Patrícia Oliveira; Maren Voglstaetter; Domitille Schvartz; Andreas R. Thomsen; Nadia Walter; Richa Khanduri; Jean‐Charles Sanchez; Andreas Keller; Carla Oliveira; Irina Nazarenko
• Keywords: Extracellular Vesicles; Extracellular vesicles

The success of malignant tumors is conditioned by the intercellular communication between tumor cells and their microenvironment, with extracellular vesicles (EVs) acting as main mediators. While value 3D conditions to study well established, impact cellular architecture on EV content function not investigated yet. Here, a recently developed cell culture microwell array adapted for production comprehensive comparative analysis biochemical features, RNA proteomic profiles EVs secreted 2D vs cultures gastric cancer cells, performed. are significantly more efficient in producing than cultures. Global upregulation microRNAs downregulation proteins observed, indicating dynamic coregulation response architecture, ADP-ribosylation factor 6 signaling pathway downregulated EVs. data strengthen biological relevance cargo