3D‐Printed Soft Lithography for Complex Compartmentalized Microfluidic Neural Devices

• DOI: 10.1002/advs.202001150
• Publication Date: 2020-06-15T08:16:58Z
• Authors: Janko Kajtez; Sebastian Buchmann; Shashank Vasudevan; Marcella Birtele; Stefano Rocchetti; Christian Jonatha...; Arto Heiskanen; Roger A. Barker; Alberto Martínez‐...; Malin Parmar; Johan Ulrik Lind; Jenny Emnéus
• Keywords: Soft Lithography; Rapid Prototyping

Compartmentalized microfluidic platforms are an invaluable tool in neuroscience research. However, harnessing the full potential of this technology remains hindered by lack a simple fabrication approach for creation intricate device architectures with high-aspect ratio features. Here, hybrid additive manufacturing is presented open-well compartmentalized neural devices that provides larger freedom design, removes need manual postprocessing, and allows increase biocompatibility system. Suitability method multimaterial integration to tailor architecture long-term maintenance healthy human stem-cell derived neurons astrocytes, spanning at least 40 days. Leveraging fast-prototyping capabilities both micro macroscale, proof-of-principle vitro model nigrostriatal pathway created. By presenting route novel materials unique systems, new possibilities biological research beyond applications.