Multimodal Imaging with NanoGd Reveals Spatiotemporal Features of Neuroinflammation after Experimental Stroke

• DOI: 10.1002/advs.202101433
• Publication Date: 2021-07-02T04:20:21Z
• Authors: Violaine Hubert; Ines Hristovska; Szilvia Karpati; Sarah Benkeder; Arindam Dey; Chloé Dumot; Camille Amaz; Naura Chounlamountri; Chantal Watrin; Jean‐Christophe C...; Fabien Chauveau; Emmanuel Brun; Patrice Marche; Fréderic Lerouge; Stéphane Parola; Yves Berthezène; Thomas Vorup‐Jensen; Olivier Pascual; Marlène Wiart
• Keywords: Internalization; Stroke; Intravital microscopy

The purpose of this study is to propose and validate a preclinical in vivo magnetic resonance imaging (MRI) tool monitor neuroinflammation following ischemic stroke, based on injection novel multimodal nanoprobe, NanoGd, specifically designed for internalization by phagocytic cells. First, it verified that NanoGd efficiently internalized microglia vitro. In MRI coupled with intravenous permanent middle cerebral artery occlusion mouse model results hypointense signals the lesion. these mice, longitudinal two-photon intravital microscopy shows activated CX3CR1-GFP/+ Ex analysis, including phase contrast synchrotron X-ray, histochemistry, transmission electron corroborate accumulation within lesion uptake immune Taken together, confirm potential NanoGd-enhanced as an biomarker at subacute stage stroke. As far known, work first decipher working mechanism MR induced nanoparticle passively targeted cells performing back-to-back MRI. Furthermore, using gadolinium-based rather than iron-based agent raises future perspectives development molecular emerging computed tomography technologies.