A 3D‐Bioprinted Functional Module Based on Decellularized Extracellular Matrix Bioink for Periodontal Regeneration

• DOI: 10.1002/advs.202205041
• Publication Date: 2022-12-14T19:38:05Z
• Authors: Xueting Yang; Yue Ma; Xiuting Wang; Shengmeng Yuan; Fangjun Huo; Genzheng Yi; Jingyi Zhang; Bo Yang; Weidong Tian
• Keywords: Decellularization; 3D bioprinting; Matrix (chemical analysis)

Abstract Poor fiber orientation and mismatched bone–ligament interface fusion have plagued the regeneration of periodontal defects by cell‐based scaffolds. A 3D bioprinted biomimetic module is designed with high architectural integrity using a methacrylate gelatin/decellularized extracellular matrix (GelMA/dECM) cell‐laden bioink. The presents favorable mechanical properties guidance high‐precision topographical cues provides biochemical environment conducive to regulating encapsulated cell behavior. dECM features robust immunomodulatory activity, reducing release proinflammatory factors M1 macrophages decreasing local inflammation in Sprague Dawley rats. In clinically relevant critical‐size defect model, significantly enhances hybrid tissues beagles, especially anchoring structures interface, well‐aligned fibers, highly mineralized alveolar bone. This demonstrates effectiveness feasibility bioprinting combined dental follicle‐specific bioink for periodontium regeneration, providing new avenues future clinical practice.