Enlarging the Stokes Shift by Weakening the π‐Conjugation of Cyanines for High Signal‐to‐Noise Ratiometric Imaging

• DOI: 10.1002/advs.202205080
• Publication Date: 2022-11-25T02:50:36Z
• Authors: Yongkang Yue; Tingting Zhao; Zhou Xu; Weijie Chi; Xiaojun Chai; Jiahong Ai; Jiawei Zhang; Fangjun Huo; Robert M. Strongin; Caixia Yin
• Keywords: Cyanine; Stokes shift; Acceptor

The signal-to-noise ratio (SNR) is one of the key features a fluorescent probe and that often defines its potential utility for in vivo labeling analyte detection applications. Here, it reported introducing pyridine group into traditional cyanine-7 dyes an asymmetric manner provides series tunable NIR (Cy-Mu-7) characterized by enhanced Stokes shifts (≈230 nm) compared to parent cyanine 7 dye (<25 nm). observed shift increase ascribed symmetry breaking Cy-Mu-7 core reduction extent conjugation. fluorescence signals are upon confinement within hydrophobic cavity albumin or via spontaneous encapsulation micelles aqueous media. Utilizing Cy-Mu-7, ultra-fast kidney mice realized, found liver injury will aggravate burden monitoring intensity liver. In addition, could be used as efficient chemiluminescence resonance energy transfer acceptor reaction between H