The SINA1‐BSD1 Module Regulates Vegetative Growth Involving Gibberellin Biosynthesis in Tomato

• DOI: 10.1002/advs.202400995
• Publication Date: 2024-08-27T16:40:40Z
• Authors: Yulin Yuan; Youhong Fan; Li Huang; Han Lu; Bowen Tan; Chloe Ramirez; Chao Xia; Xiangli Niu; Sixue Chen; Mingjun Gao; Cankui Zhang; Yongsheng Liu; Fangming Xiao
• Keywords: Dwarfism; Genetically modified tomato

In plants, vegetative growth is controlled by synergistic and/or antagonistic effects of many regulatory factors. Here, the authors demonstrate that ubiquitin ligase seven in absentia1 (SINA1) mammalian BTF2-like transcription factors, Drosophila synapse-associated proteins, and yeast DOS2-like proteins (BSD1) function as a module to control tomato via regulation production plant hormone gibberellin (GA). SINA1 negatively regulates protein level BSD1 through ubiquitin-proteasome-mediated degradation, transgenic over-expressing (SINA1-OX) resembles dwarfism phenotype BSD1-knockout (BSD1-KO) plant. directly activates expression BSD1-regulated gene 1 (BRG1) binding novel core BBS (standing for site) motif BRG1 promoter. Knockout (BRG1-KO) also results phenotype, suggesting plays positive role does. Significantly, GA contents are attenuated SINA1-OX, BSD1-KO, BRG1-KO plants exhibiting exogenous application bioactive