Mild Hyperthermia Accelerates Bone Repair by Dynamically Regulating iNOS/Arg1 Balance in the Early Stage

• DOI: 10.1002/advs.202409882
• Publication Date: 2024-12-31T12:38:32Z
• Authors: Jinhui Zhao; Yiping Luo; Lei Zhang; Yunfeng Chen; Yixing Chen; Xinhui Wu; Aihemaitijiang Ai...; Dilixiati Duolikun; Tianlong Wang; Zifei Zhou; Zhiqing Liu; Longpo Zheng
• Keywords: 0303 health sciences; 03 medical and health sciences; Science; Q; macrophage; iNOS/Arg1 balance; bone repair; mild hyperthermia

Mild hyperthermia therapy has garnered interest as an adjunctive treatment for bone repair. However, its optimal timing, duration, and underlying mechanisms remain unclear. In this study, how mild supports repair during the early stages is assesed. These findings reveal that accelerates regeneration by dynamically regulating inducible nitric oxide synthase/arginase 1 (iNOS/Arg1) balance. This process involves macrophage polarization to M1 phenotype through iNOS activation, followed a rapid transition M2 Arg1 activation after 3 days of sustained hyperthermia. RNA-Seq reveals single day induced immune alterations aligned with inflammatory phase repair, characterized osteoclast cell recruitment, neovascularization, thereby preparing phase. Experiments involving subcutaneous abscesses, embedding, critical cranial defects further confirm regulates phenotypes. regulation enhances antibacterial activity, promotes angiogenesis, facilitates from inflammation ultimately accelerating bone-defect study first elucidate dual temporal effects on regulation, offering insights into timing duration photothermal following surgery.