X‐Ray Structure and Designed Evolution of an Artificial Transfer Hydrogenase

• DOI: 10.1002/anie.200704865
• Publication Date: 2008-01-04T15:19:03Z
• Authors: Marc Creus; Anca Pordea; Thibaud Rossel; Alessia Sardo; Christophe Letondor; Anita Ivanova; Isolde LeTrong; Ronald E. Stenkamp; Thomas R. Ward
• Keywords: Hydrogenase; Coordination sphere; Directed Molecular Evolution

A structure is worth a thousand words: Guided by the X-ray of an S-selective artificial transfer hydrogenase, designed evolution was used to optimize selectivity hybrid catalysts. Fine-tuning second coordination sphere ruthenium center (see picture, orange sphere) introduction two point mutations allowed identification selective hydrogenases for reduction dialkyl ketones.