Reactive and Bioactive Cationic α‐Helical Polypeptide Template for Nonviral Gene Delivery

• DOI: 10.1002/anie.201104262
• Publication Date: 2011-12-07T14:56:22Z
• Authors: Nathan P. Gabrielson; Hua Lu; Lichen Yin; Dong Li; Fei Wang; Jianjun Cheng
• Keywords: Molecular Structure; Genetic Vectors; Gene Transfer Techniques; Genetic Therapy; 02 engineering and technology; Protein Structure, Secondary; Cations; COS Cells; Chlorocebus aethiops; Animals; Humans; Peptides; 0210 nano-technology

At every turn: Poly(γ-(4-vinylbenzyl)-L-glutamate) (PVBLG) is a bioactive and reactive template for library of cationic α-helical polypeptides (PVBLGn-X) gene delivery. The top-performing PVBLGn-X outperformed polyethyleneimine (PEI) by 12-fold. Preliminary data indicate that the helicity essential their performance, enhanced membrane disruption likely source transfection efficiency. RLU=relative light unit.