Batch, fed‐batch, and microcarrier cultures with CHO cell lines in a pressure‐cycle driven miniaturized bioreactor

• DOI: 10.1002/bit.23289
• Publication Date: 2011-09-30T16:45:26Z
• Authors: Beum Jun Kim; Ti Zhao; Lincoln Young; Peng Zhou; Michael L. Shuler
• Keywords: Microcarrier

Miniaturized bioreactors for suspension cultures of animal cells, such as Chinese Hamster Ovary (CHO) could improve bioprocess development through the ability to cheaply explore a wide range operating conditions. A miniaturized pressure-cycled bioreactor cell cultures, described previously (Diao et al., 2008), was tested with CHO line producing commercially relevant quantities human IgG. Results from suspended showed that growth comparable conventional flask controls and target protein production enhanced in minibioreactor, which may be due relatively high oxygen transfer rate moderate shear stress, measured simulated previously. Microcarrier culture using an anchorage-dependent Cytodex 3 also similar result: model (secreted alkaline phosphatase or SEAP). Various fed-batch schemes were applied cells IgG, yielding numbers (1.1 × 10(7) /mL) at day 8 titers IgG (2.3 g/L) 14 are typical industrial values cultures. The alteration volumetric coefficient is key parameter viability We conclude minibioreactor can provide favorable environments; mixing time altered mimic larger scale system allowing potential prediction response during scale-up.