Collagen type IV‐specific tripeptides for selective adhesion of endothelial and smooth muscle cells

• DOI: 10.1002/bit.24459
• Publication Date: 2012-02-13T13:37:34Z
• Authors: Kei Kanie; Yuji Narita; Yingzi Zhao; Fumiaki Kuwabara; Makoto Satake; Susumu Honda; Hiroaki Kaneko; Tomohiko Yoshioka; Mina Okochi; Hiroyuki Honda; Ryuji Kato
• Keywords: Tripeptide; Type I collagen

Controlling the balance of endothelial cells (ECs) and smooth muscle (SMCs) in blood vessels is critically important to minimize risk associated with vascular implants. Extracellular matrix (ECM) plays a key role controlling cellular balance, suggesting promising source cell-selective peptides. To obtain EC- or SMC-selective peptides, we start by highlighting sequence differences found among ECM molecules as enriched targets for We explored performance tripeptides that are specifically only collagen type IV, but not types I, II, III, V. Collagen IV was chosen since it major basement membrane vessels, which separates ECs SMCs. Among 114 IV-derived pre-screened from silico analysis, 22 peptides (19%) were promote adhesion, determined peptide array. One best performing EC-selective (Cys-Ala-Gly (CAG)) mixed into an electrospun fine-fiber, graft material, practical application. Compared unmodified fiber, CAG containing fiber surface enhance adhesion (+190%) while limiting SMCs (-20%). These results consistent hypothesis cell selective also suggest new genre tissue engineering applications. Collectively, these findings favorably support screening approach used discover purposes.