Simultaneous quantification of oleuropein and its metabolites in rat plasma by liquid chromatography electrospray ionization tandem mass spectrometry

• DOI: 10.1002/bmc.1319
• Publication Date: 2009-09-30T09:55:18Z
• Authors: Fotini N. Bazoti; Evangelos Gikas; Anthony Tsarbopoulos
• Keywords: Hydroxytyrosol; Oleuropein

Abstract Oleuropein (OE) is the cardinal bioactive compound derived from Olea europaea and possesses numerous beneficial properties for human health. However, despite plethora of analytical methods that have studied biological fate olive oil‐derived compounds, no validated methodology has been published to date simultaneous determination OE, along with all its major metabolites. In this study, a liquid chromatography‐electrospray ionization‐tandem mass spectrometry (LC‐ESI MS/MS) method developed quantification simultaneously main metabolites hydroxytyrosol, 2‐(3,4‐dihydroxyphenyl)acetic acid, 4‐(2‐hydroxyethyl)‐2‐methoxy‐phenol or homovanillyl alcohol, 2‐(4‐hydroxy‐3‐methoxyphenyl)acetic acid homovanillic elenolic in rat plasma matrix. Samples were analyzed by LC‐ESI MS/MS prior after enzymatic treatment. A solid‐phase extraction step high mean recovery compounds was performed as sample pretreatment. Calibration curves linear over range studied, while exhibited good accuracy, intra‐ inter‐day precision. The limit detection picogram (per milliliterof plasma) HT OE nanogram milliliter other analytes, simple rapid. successfully applied aforementioned samples, thus demonstrating suitability pharmacokinetics, well bioavailability metabolism studies. Copyright © 2009 John Wiley & Sons, Ltd.