New Pyranopyrazole‐Based Indolin‐2,3‐Dione Hybrid as Effective Inhibitors of Xanthine Oxidase: Synthesis, In Vitro, and Molecular Modeling Approaches

• DOI: 10.1002/cbdv.202402104
• Publication Date: 2025-01-08T08:29:04Z
• Authors: Osama Alharbi; Khalid Awadh Al‐M...; Munjed M. Ibrahim; Ramith Ramu; Mohammed Al‐Ghorbani
• Keywords: Ethyl acetoacetate; IC50; Docking (animal); Condensation reaction

In the current study, new pyranopyrazole analogues (9a–d and 10a–d) were synthesized through a one‐pot condensation reaction of 2‐arylacetohydrazide. The inhibitory abilities investigated against XO enzyme experimental molecular docking analyses. synthesis studies based on ultrasound‐mediated reactions four‐component systems containing 2‐arylacetohydrazide, ethyl acetoacetate, indoline‐2,3‐dione, 2‐cyanoacetate/malononitrile in various solvents catalysts to yield short time remarkably favorable yields ranging from 79–92%. Based inhibition study compounds 9a‐d 10a‐d, compound 10d was most potent (IC50 = 0.09 ± 0.22 µM), followed by 9c (0.12 0.11 µM). With IC50 values 0.20 0.27 µM 0.17 respectively, 10a 10c exhibited moderate activity. other have shown less activity compared allopurinol control 0.14 0.10 Furthermore, analysis, predicted highest binding affinity target enzyme.