Nonlinear Impact of Electrolyte Solutions on Protein Dynamics

• DOI: 10.1002/cbic.202400057
• Publication Date: 2024-02-23T09:20:49Z
• Authors: Hosein Geraili Da...; Benedikt Söldner; Himanshu Singh; Rasmus Linser; Ana Vila Verde
• Keywords: Dynamics

Abstract Halophilic organisms have adapted to multi‐molar salt concentrations, their cytoplasmic proteins functioning despite stronger attraction between hydrophobic groups. These proteins, of interest in biotechnology because decreasing fresh‐water resources, excess acidic amino acids. It has been suggested that conformational fluctuations – critical for protein function decrease the presence a effect, and an proteome would counteract this decrease. However, our understanding salt‐ acid dependency enzymatic activity is limited. Here, using solution NMR relaxation molecular dynamics simulations total 14 we show concentration limited moreover non‐monotonic impact on dynamics. The results speak against conformational‐fluctuations model, instead indicating maintaining ensure not evolutionary driving force behind halophilic proteins.