Biocatalytic Reductions and Chemical Versatility of the Old Yellow Enzyme Family of Flavoprotein Oxidoreductases

• DOI: 10.1002/cctc.201000094
• Publication Date: 2010-06-28T18:13:17Z
• Authors: Helen S. Toogood; John M. Gardiner; Nigel S. Scrutton
• Keywords: Biocatalysis; Flavoprotein; Stereospecificity; Flavin mononucleotide

Abstract The old yellow enzyme (OYE) family is a large group of flavin‐dependent redox biocatalysts with major applications in the industrial reduction activated alkenes. These enzymes have broad specificity, are relatively stable, and been made available quantities by using conventional genetic methods. catalytic cycle comprises two half‐reactions: flavin mononucleotide NAD(P)H followed oxidation through stereospecific CC bond wide range Recent years witnessed extensive investigation these reactions, aided knowledge atomic resolution structures for selected members. In turn, this has led to deep understanding stereochemical course substrate expansion biocatalytic versatility engineering approaches. We provide an overview structures, mechanisms, chemical specificity reactions catalyzed OYE potential illustrate value combining mechanistic structural studies guide future exploitation biocatalysis.