Time‐resolved fluorescent chirality sensing and imaging of malate in aqueous solution

• DOI: 10.1002/chir.20185
• Publication Date: 2005-08-15T19:46:59Z
• Authors: Zhihong Lin; Meng Wu; Otto S. Wolfbeis
• Keywords: Malates; Water; Hydrogen-Ion Concentration; 01 natural sciences; Time; 0104 chemical sciences; Kinetics; Spectrometry, Fluorescence; Europium; 540 Chemie; enantiomeric discrimination; time-resolved fluorescence; europium chiral sensing; imaging; malate; Fluorescent Dyes

AbstractChiral discrimination of malates in aqueous solutions at near‐neutral pH is achieved through fluorescence measurement and imaging using the europium–tetracycline complex (EuTc) as a fluorescent probe. The method is based on the significantly different fluorescence properties of the ternary complexes (Eu–Tc–malate) formed between EuTc and the enantiomeric malates. The enantiomeric excess (ee) of chiral malates can be quantified by both steady‐state and time‐resolved fluorescence, using either a conventional fluorescence microplate reader or fluorescence imaging. It offers a facile and sensitive method for high‐throughput chiral discrimination. © 2005 Wiley‐Liss, Inc. Chirality 17:464–469, 2005.