Chronic Imaging of Mouse Brain: From Optical Systems to Functional Ultrasound

• DOI: 10.1002/cpns.98
• Publication Date: 2020-06-25T22:03:45Z
• Authors: Kıvılcım Kılıç; Jianbo Tang; Ş. Evren Erdener; Smrithi Sunil; John T. Giblin; Blaire S. Lee; Dmitry D. Postnov; Anderson Chen; David A. Boas
• Keywords: 0301 basic medicine; Mice; 03 medical and health sciences; Functional Neuroimaging; Optical Imaging; Neurosciences; Animals; Brain; Ultrasonography; 3. Good health

AbstractUtilization of functional ultrasound (fUS) in cerebral vascular imaging is gaining popularity among neuroscientists. In this article, we describe a chronic surgical preparation method that allows longitudinal studies and therefore is applicable to a wide range of studies, especially on aging, stroke, and neurodegenerative diseases. This method can also be used with awake mice; hence, the deleterious effects of anesthesia on neurovascular responses can be avoided. In addition to fUS imaging, this surgical preparation allows researchers to take advantage of common optical imaging methods to acquire complementary datasets to help increase the technical rigor of studies. © 2020 Wiley Periodicals LLC.Basic Protocol 1: Surgical preparation of mouse chronic cranial windows using polymethylpenteneBasic Protocol 2: Imaging of mice with chronic cranial windows