Pachytene Chromosome Preparation in Populus deltoides Marsh

• DOI: 10.1002/cppb.20036
• Publication Date: 2016-12-14T20:01:19Z
• Authors: Haoyang Xin; Yue Lan; Jisen Shi; Yan Ma; Mengli Xi
• Keywords: 0301 basic medicine; 03 medical and health sciences

Abstract A technique that produces large numbers of good quality pachytene chromosome preparations has been developed for Populus . Anthers at the stage meiosis are used as materials. There two main modifications in our method relative to traditional squashing address challenges thick cytoplasm observed during phase presents. One is temperature squashing, i.e., slide placed on a 52°C heater squashing. The other removal cover slip using 45% acetic acid. © 2016 by John Wiley & Sons, Inc.