Rapid method for the simultaneous determination of DDTs and PCBs in hair of children by headspace solid phase microextraction and gas chromatography‐mass spectrometry (HSSPME/GC‐MS)

• DOI: 10.1002/dta.1631
• Publication Date: 2014-05-09T12:42:49Z
• Authors: Manolis N. Tzatza...; Emmanouil G. Barb...; Matthaios P. Kavv...; Elena Vakonaki; Elisavet Renieri; Alexander I. Vard...; Aristidis M. Tsat...
• Keywords: Solid-Phase Microextraction; Solid phase extraction

The purpose of this study was to develop a rapid and cost efficient hair extraction method, using the headspace solid phase microextraction (HSSPME) technique for simultaneous determination biomonitoring 1,1,1‐trichloro‐2,2‐bis(4‐chlorophenyl)ethane) (DDT) its isomers/metabolites polychlorinated biphenyls (PCBs) in samples. A total 72 head samples were collected from children living urban rural regions island Crete. Two hundred milligrams digested under alkaline conditions thermostated 30 min at 90°C while 65 µm PDMS/DVB fibre exposed into vial. Analytical parameters method (time incubation, agitation speed, recovery, precision, accuracy, carry over, matrix effect, linearity, selectivity) examined. Recoveries DDTs spiked calculated 42.3% opDDD 87.1% opDDE, recoveries PCB congeners 52.6% PCB138 96.6 % PCB28. applied analysis authentic Significant differences (p=0.001) burden (sumDDTs) as well frequencies detection positive (p=0.020) observed between examined regions. Moreover, significant detected concentrations PCB52 (p<0.001) PCB28 (p=0.017) their prevalence Application HSSPME PCBs biomarkers tested successfully found be substantially simpler faster procedure than previous reported sample treatment procedures. Copyright © 2014 John Wiley & Sons, Ltd.