Three different active aldehydes induce the production of advanced lipoxidation end products upon incubation with bovine serum albumin

• DOI: 10.1002/ejlt.201400339
• Publication Date: 2015-02-24T06:51:34Z
• Authors: Wenjuan Li; Haiyan Gao; Honglei Mu; Hangjun Chen; Xiangjun Fang; Yongjun Zhou; Fei Tao
• Keywords: Bovine serum albumin; Lipofuscin; Absorbance; Malondialdehyde; Sodium dodecyl sulfate; Serum Albumin

Accumulation of advanced lipoxidation end products (ALEs) has been implicated in many chronic and degenerative diseases. In this study, we evaluated the effects malondialdehyde (MDA), trans ‐2‐heptenal, , ‐2,4‐heptadienal on properties native proteins formation bovine serum albumin (BSA)–ALEs. Incubation BSA with these aldehydes resulted modification Lys Arg residues, conformational changes, colored, fluorescent, lipofuscin‐like pigments (exhibiting strong fluorescence for MDA–BSA, but much weaker other two aldehyde–BSA molecules). Moreover, generated BSA–aldehyde complexes exhibited ultraviolet–visible (UV–Vis) absorption, i.e., MDA–BSA adduct increased UV–Vis absorbance at 290 400 nm, while molecules 280 300–350 respectively, cross‐linking, similar to characteristics Maillard reaction products. The aggregates was detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis. variables measured above showed that heptadienal–BSA heptenal–BSA samples both similarities differences from samples. These results provide insights into ALEs terms their biochemical interactions applications food science. Practical applications: Advanced are known be involved Here, propose suitable protocols characterize compare derived different secondary lipid oxidation. enhance our knowledge contribute understanding structure oxidation Additionally, important appropriate preventive or therapeutic measures inhibit reduce ALEs. MDA, heptenal, heptadienal conformation generation exhibiting AGEs (Maillard products).