Oxidized Lipids Decrease Phagocytic Activity of ARPE‐19 Cells In Vitro

• DOI: 10.1002/ejlt.201800476
• Publication Date: 2019-05-17T09:46:05Z
• Authors: Anna M. Pawlak; Magdalena Olchawa; Anna Koscielniak; Andrzej Zadlo; Agnieszka Broniec; Tomasz Oles; Tadeusz J. Sarna
• Keywords: Phototoxicity; Lipid Oxidation

Phospholipids, representing 80% of retinal lipids, contain a high level polyunsaturated fatty acids, which are susceptible to oxidation. It has recently been shown that Folch’ extract bovine neural retinas reveals moderate photoreactivity by generating reactive oxygen species upon irradiation with blue light. Importantly, the markedly increases oxidation exctracted lipids. In this work, phototoxic potential (BRex), subjected controlled oxidation, is studied in vitro employing pigment epithelium cell line (ARPE‐19). Multilamellar liposomes prepared from BRex, equilibrated air, oxidized dark at 37 °C for up 400 h. The then introduced into ARPE‐19 cells phagocytosis. impact BRex different stages on viability and specific phagocytic activity exposed visible light analyzed. While doses employed, does not induce any measurable killing cells, treatment noticeably decreases their activity. Practical Applications : Lipid products commonly considered as an efficient protein modification factor occurring retina. Although possible consequences lipids onset development age‐related macular degeneration widely discussed, phototoxicity have considered. This important issue; taking account lipid present outer retina, it may be short‐wavelength radiation. If photoreactive, peroxidized act acquired endogenous sensitizers increasing risk retina photodamage. Lipids characterized unsaturation makes them When oxidized, become photoreactive under Treatment (ARPE‐19) followed light, reduces cells.