Characterizing the Pulmonary Toxicity and Potential Mutagenicity of Formaldehyde Fumes in a Human Bronchial Epithelial Tissue Model

• DOI: 10.1002/em.70000
• Publication Date: 2025-03-11T13:01:13Z
• Authors: Yuan Le; Baiping Ren; Levan Muskhelishvili; Kelly Davis; Yiying Wang; William Gwinn; Diego Rua; Robert H. Heflich; Xuefei Cao
• Keywords: Pulmonary toxicity

ABSTRACT Formaldehyde (FA) is a highly reactive aldehyde that regarded as an inhalation hazard and human carcinogen. Herein, we report follow‐up study evaluating the effects of exposure duration on toxicity mutagenicity FA using in vitro air‐liquid‐interface (ALI) airway tissue model. Previously exposed ALI cultures to 7.5, 15 30‐ppm fumes 4 h/day for 5 days; currently, have increased 7.5 ppm days/week weeks, followed by 28‐day recovery. Due its toxicity, 30 were treated days, Tissue responses evaluated following treatment DNA damage was measured Comet‐Chip assay after 3 days exposure, mutagenesis duplex sequencing The detected 4‐week similar seen previously with 5‐day exposures: both induced moderate decreases integrity, FANCD2 DNA‐repair enzyme expression IL‐6 release, increases IL‐1RA release. Effects cell proliferation, ciliary function structure minimal. Additionally, neither nor or mutations. Using this experimental design, does not produce genotoxicity mutagenicity, even when exposures are conducted over period.