Deciphering the contribution of PerR to oxidative stress defense system in Clostridium tyrobutyricum

• DOI: 10.1002/fft2.205
• Publication Date: 2023-01-07T05:41:30Z
• Authors: Lei Yang; Zhihan Yang; Jiayu Liu; Zilong Liu; Yuxin Liu; Liying Zhu; Zhengming Zhu; Ling Jiang
• Keywords: Clostridium acetobutylicum

Abstract As a next‐generation probiotic, Clostridium tyrobutyricum , which is considered obligate anaerobe, has been extensively promising candidate for future use to promote health benefits. The industrial applications were limited by its weak tolerance oxygen stress during fermentation. Here, we enhanced the oxidative of C. L319 deleting peroxide response regulator gene perR using an endogenous CRISPR‐Cas system. mutant strain exhibited greatly improved growth when exposed oxygen, also showed shortened lag phase compared with control strain. Consistently, concomitant increase in butyric acid productivity from 0.08 0.13 g/L/h presence was observed. Decreased intracellular ROS level sand NAD + /NADH ratios observed Furthermore, transcriptome and binding target analysis revealed that genes encoding transcriptional regulators proteins related redox balance upregulated. Changes carbohydrate, amino purine metabolisms upon exposure Importantly, phylogenomic indicated PerR may be universal engineering diverse species. These findings will contribute development robust strains efficient production food industry.