Bone morphogenetic protein 4 regulates microRNA expression in breast cancer cell lines in diverse fashion

• DOI: 10.1002/gcc.22324
• Publication Date: 2015-12-19T02:41:37Z
• Authors: Emma‐Leena Alarmo; Riikka Havunen; Sergei Häyrynen; Sanna Penkki; Johanna Ketolainen; Matti Nykter; Anne Kallioniemi
• Keywords: 0301 basic medicine; MicroRNAs; 03 medical and health sciences; Cell Movement; Cell Line, Tumor; Humans; Breast Neoplasms; Female; Bone Morphogenetic Protein 4; 3. Good health; Cell Proliferation

Bone morphogenetic protein 4 (BMP4) is a remarkably powerful inhibitor of breast cancer cell proliferation, but it also able to induce migration in certain cellular contexts. Previous data demonstrate that BMP4 controls the transcription variety protein‐coding genes, not much known about microRNAs (miRNA) regulated by BMP4. To address this question, miRNA expression profiles following treatment were determined one mammary epithelial and seven lines using microarrays. While analysis revealed an extensive variation differentially expressed across lines, four miRNAs (miR‐16‐5p, miR‐106b‐5p, miR‐23a‐3p, miR‐23b‐3p) commonly induced subset cells upon treatment. Inhibition their demonstrated increase BT‐474 number, indicating they possess tumor suppressive properties. However, with exception these effects independent Scratch assay miR‐16‐5p miR‐106b‐5p inhibitors on BMP4‐treated MDA‐MB‐231 resulted enhanced migration, suggesting are engaged BMP4‐induced motility. Taken together, we have for first time characterized showing contribute fine‐tuning proliferation phenotypes. © 2015 Wiley Periodicals, Inc.