Frequent BRG1/SMARCA4-inactivating mutations in human lung cancer cell lines

• DOI: 10.1002/humu.20730
• Publication Date: 2008-04-03T16:19:12Z
• Authors: Pedro P. Medina; Octavio A. Romero; Takashi Kohno; Luis M. Montuenga; Ruben Pio; Jun Yokota; Montse Sanchez-Ce...
• Keywords: SMARCA4; SWI/SNF

Components of the SWI/SNF chromatin-remodeling complex, such as INI1, are inactivated in human cancer and, thus, act tumor suppressors. Here we screened for mutations entire coding sequence BRG1 (SMARCA4), which encodes ATPase 59 lung cell lines most common histopathological types. Mutations were detected 24% lines, many them cells commonly used research. All homozygous and predicted truncated proteins. The alterations significantly more frequent non-small-cell (NSCLC) type (13/37, 35%) compared to small-cell (SCLC) (1/19, 5%) (P<0.05; Fisher's Exact test) was fourth frequently altered gene NSCLC lines. coexisted with mutations/deletions at KRAS, LKB1, NRAS, P16, P53. However, always occurred absence MYC amplification, suggesting a role development. In conclusion, our data strongly support that is bona fide suppressor major factor tumorigenesis. Hum Mutat 29(5), 617–622, 2008. © 2008 Wiley-Liss, Inc.