Vitamin D improves autoimmune diseases by inhibiting Wnt signaling pathway

• DOI: 10.1002/iid3.1192
• Publication Date: 2024-02-28T07:04:35Z
• Authors: Minshu Zou; Qiuju Song; Taiyong Yin; Hongtao Xu; Guoming Nie
• Keywords: 0303 health sciences; 03 medical and health sciences; Wnt/β‐catenin signal pathway; autoimmune diseases; vitamin D; Original Articles; Immunologic diseases. Allergy; RC581-607

Abstract Objective In this study, we investigated the development of Wnt signaling pathway in vitamin D (VitD) to improve systemic lupus erythematosus mice breakthrough clinical treatment approaches. Methods Body weight changes were recorded during rearing. Antinuclear antibodies (ANA), anti‐dsDNA, and anti‐snRNP detected mouse serum using an enzyme‐linked immunosorbent assay. Apoptosis Th1 Th2 immune cells was flow cytometry. Reverse transcription polymerase chain reaction used detect expression T‐bet, GATA3, Wnt3a mRNA spleens each group. Western blot analysis performed Wnt1, p‐β‐catenin, β‐catenin, glycogen synthase kinsase3β (GSK‐3β), Wnt3a, c‐myc, cyclin D1 protein spleens. β‐catenin spleen visualized immunohistochemistry. Results VitD did not substantial reduce body MRL/LPR mice, whereas inhibitor did. notably decreased concentrations ANA, anti‐double‐stranded DNA, alleviated apoptosis cells. markedly increased T‐bet GATA consequently levels β‐catenin. revealed that GSK‐3β, could be reduced by VitD, compared with MRL/LPR. Immunohistochemistry demonstrated most pronounced level substantially after intervention. Conclusions can further inhibit nuclear translocation downregulating ligands (Wnt1 Wnt3a), which reduces downstream target gene D1. Systemic improved inhibiting activation Wnt/β‐catenin signal pathway.