Lactobacillus rhamnosus GG triggers intestinal epithelium injury in zebrafish revealing host dependent beneficial effects

• DOI: 10.1002/imt2.181
• Publication Date: 2024-03-26T06:17:08Z
• Authors: Zhen Zhang; Hong‐Ling Zhang; Da‐Hai Yang; Qiang Hao; Hong‐Wei Yang; De‐Long Meng; Willem Meindert d...; Le‐Luo Guan; Shu‐Bin Liu; Tsegay Teame; Chen‐Chen Gao; Chao Ran; Ya‐Lin Yang; Yuan‐Yuan Yao; Qian‐Wen Ding; Zhi‐Gang Zhou
• Keywords: Pyroptosis; Lactobacillus rhamnosus; Intestinal epithelium; Dysbiosis

Abstract Lactobacillus rhamnosus GG (LGG), the well‐characterized human‐derived probiotic strain, possesses excellent properties in maintenance of intestinal homeostasis, immunoregulation and defense against gastrointestinal pathogens mammals. Here, we demonstrate that SpaC pilin LGG causes epithelium injury by inducing cell pyroptosis gut microbial dysbiosis zebrafish. Dietary activates Caspase‐3−GSDMEa pathways epithelium, promotes increases lipopolysaccharide (LPS)‐producing microbes The increased LPS subsequently Gaspy2−GSDMEb pathway. Further analysis reveals is initiated species‐specific recognition TLR4ba, which accounts for species‐specificity SpaC‐inducing observed pyroptosis‐driven zebrafish suggest host‐specific beneficial/harmful mechanisms are critical safety issues when applying probiotics derived from other host species need more attention.