Oxidative stress‐mediated apoptosis is involved in bisphenol S‐induced reproductive toxicity in male C57BL/6 mice

• DOI: 10.1002/jat.4170
• Publication Date: 2021-05-18T01:22:21Z
• Authors: Wei Dai; Qing‐zhi He; Bi‐qi Zhu; Huai‐cai Zeng
• Keywords: Reproductive toxicity; Fas ligand

The reproductive toxicity of bisphenol S (BPS) in male mammals and its possible mechanism are not clear. We investigated the effects action BPS on adult C57BL/6 mice. found that exposure to 200-mg/kg resulted a significant decrease sperm count caput/corpus cauda epididymis, significantly decreased motility, increased deformity. Histological evaluation revealed caused spermatozoa lumen seminiferous tubules reduction proportion Stage VII or VIII BPS-treated groups. Furthermore, ultrastructure analysis BPS-induced mitochondrial damage apoptosis spermatogenic cells. Moreover, exposure-induced oxidative stress testicular tissues. Further, dUTP-biotin nick end labeling (TUNEL) assay showed induced cells dose-dependent manner. also upregulated cleaved caspase-8, caspase-9, caspase-3, Fas, FasL downregulated Bcl-2/Bax ratio. These results suggest testis cell potentially impairs spermatogenesis function, which may be BPS. Fas/FasL signal pathways involved stress-related apoptosis.