The use of FTIR spectroscopy to assess quantitative changes in the biochemical composition of microalgae

• DOI: 10.1002/jbio.201000019
• Publication Date: 2010-05-27T02:31:10Z
• Authors: Heiko Wagner; Zhixin Liu; Uwe Langner; Katja Stehfest; Christian Wilhelm
• Keywords: 0106 biological sciences; Bacterial Proteins; Spectroscopy, Fourier Transform Infrared; Microalgae; 01 natural sciences; Chlamydomonas reinhardtii

A mid-infrared spectroscopic method was developed for the simultaneous and quantitative determination of total protein, carbohydrate lipid contents microalgal cells. Based on a chemometric approach, measured FTIR (Fourier transform infrared) spectra from algal cells were reconstructed by partial least square algorithm, using reference substances to determine their relative contribution overall cell spectrum. From this specific absorption, absolute macromolecular composition [pg cell(-1)] can be calculated calibration curves, which have been validated independent biochemical methods. The future potential photosynthesis research is shown its application follow time-resolved changes in cellular microalgae during an illumination period several hours. We show how investigated spectroscopy This substantially increase efficiency screening processes like bioreactor monitoring may beneficial metabolic engineering