Optical plasticity of mammalian cells

• DOI: 10.1002/jbio.202000457
• Publication Date: 2020-12-21T17:38:39Z
• Authors: Kaushikaram Subra...; Heike Petzold; Benjamin Seelbinder; Lena Hersemann; Ina Nüsslein; Moritz Kreysing
• Keywords: Opacity

Transparency is widespread in nature, ranging from transparent insect wings to ocular tissues that enable you read this text, and marine vertebrates. And yet, cells tissue models biology are usually strongly light scattering optically opaque, precluding deep optical microscopy. Here we describe the directed evolution of cultured mammalian toward increased transparency. We find mutations greatly diversify phenotype Chinese Hamster Ovary cells, a cell line. Furthermore, only three rounds high-throughput selection competitive growth required yield fit with improved Based on 15 monoclonal lines derived experiment, evolved transparency frequently goes along reduction nuclear granularity physiological shifts gene expression profiles. In future plasticity may facilitate genetic clearance living for vivo