Line‐field confocal optical coherence tomography as a tool for three‐dimensional in vivo quantification of healthy epidermis: A pilot study

• DOI: 10.1002/jbio.202100236
• Publication Date: 2021-10-07T05:59:20Z
• Authors: Julie Chauvel‐Picard; Vincent Bérot; Linda Tognetti; Carmen Orte Cano; Margot Fontaine; Clément Lenoir; Javiera Pérez‐Anker; Susana Puig; Arnaud Dubois; Sandra Forestier; Jilliana Monnier; Randa Jdid; Gabriel Cazorla; Mélanie Pedrazzani; Antoine Sanchez; Sébastien Fischman; Pietro Rubegni; Véronique del Marmol; Joseph Malvehy; Elisa Cinotti; Jean L. Perrot; Mariano Suppa
• Keywords: Epidermis (zoology)

Abstract Epidermal three‐dimensional (3D) topography/quantification has not been completely characterized yet. The recently developed line‐field confocal optical coherence tomography (LC‐OCT) provides real‐time, high‐resolution, in‐vivo 3D imaging of the skin. This pilot study aimed at quantifying epidermal metrics (epidermal thicknesses, dermal‐epidermal junction [DEJ] undulation and keratinocyte number/shape/size) using LC‐OCT. For each participant (8 female, skin‐type‐II, younger/older volunteers), seven body sites were imaged with calculated by segmentations measurements assisted artificial intelligence (AI) when appropriate. Thicknesses epidermis/SC, DEJ nuclei volume varied across sites. Evidence maturation was observed in vivo: being small/spherical near flatter/elliptical skin surface. Skin microanatomy can be quantified combining LC‐OCT AI. technology could highly relevant to understand aging processes conditions linked disorders. Future clinical/research applications are expected this scenario.