Multilayer diffusion modeling and Coherent anti‐Stokes Raman scattering microscopy for spatially resolved water diffusion measurements in human skin

• DOI: 10.1002/jbio.202200110
• Publication Date: 2022-07-20T06:35:06Z
• Authors: Irina Iachina; Michael A. Lomholt; Johannes H. Eriksen; Jonathan R. Brewer
• Keywords: Microscopy; Spectrum Analysis; multilayer diffusion modeling; Raman/methods; Water; water diffusion; Spectrum Analysis, Raman; 01 natural sciences; Acetone; Water/chemistry; 0103 physical sciences; Skin/diagnostic imaging; Humans; CARS; measurements; skin Barrier; Skin

In this work using Coherent anti-Stokes Raman Scattering microscopy, it was possible to directly measure the time dependent, spatially resolved change in concentration of water (D2 O) intact skin tissue with a spatial resolution under 1 μm, and combined multilayer diffusion model, coefficients at different depths were extracted. The results show that varies layers throughout Stratum Corneum (SC), indicating SC is not homogeneous barrier but complicated heterogeneous structure. Interestingly, averaging over samples gave relatively constant value 0.047 ± 0.01 μm2 /second. Treating acetone or tape stripping led an increased coefficient 0.064 0.02 /second 0.079 0.03 /second, respectively. method model presented here shows potential for wide applications measuring substances variety samples.