Investigating the Impact of Long‐Term Alcohol Consumption on Serum Chemical Changes: Fourier Transform Infrared Spectroscopy for Human Blood Serum

• DOI: 10.1002/jbio.202400550
• Publication Date: 2025-03-12T06:40:14Z
• Authors: Zozan Guleken; Devrim Sarıbal; Hasan Mırsal; Jozef Cebulski; Zeynep Ceylan; Joanna Depciuch
• Keywords: Human blood; Fourier transform spectroscopy

ABSTRACT Chronic alcohol consumption significantly impacts physiological and neurological functions. This study aimed to investigate the biochemical alterations in serum associated with long‐term use using Fourier Transform Infrared (FTIR) spectroscopy. Serum samples from control disorder (AUD) were analyzed, their spectra compared. Multivariate analysis techniques, including Principal Component Analysis (PCA) Hierarchical Cluster (HCA), employed differentiate groups. A machine learning model, Grid Search‐Support Vector Machine Discriminant (GS‐SVMDA), was developed classify high accuracy. Significant differences absorbance values of specific functional groups, particularly those phospholipids, amides, fatty acids revealed. The AUD exhibited lower levels these biomolecules. models achieved perfect classification, demonstrating potential ofFTIR spectroscopy as a non‐invasive tool for diagnosing AUD. Findings contribute better understanding mechanisms underlying addiction may aid development novel diagnostic therapeutic strategies.