Fabrication and surface modification of macroporous poly(L‐lactic acid) and poly(L‐lactic‐co‐glycolic acid) (70/30) cell scaffolds for human skin fibroblast cell culture

• DOI: 10.1002/jbm.10318
• Publication Date: 2002-09-05T20:20:51Z
• Authors: Jian Yang; Guixin Shi; Jianzhong Bei; Shenguo Wang; Yilin Cao; Qingxin Shang; Guanghui Yang; Wenjing Wang
• Keywords: Glycolic acid; Surface Modification; Anhydrous; Dermal fibroblast

Abstract The fabrication and surface modification of a porous cell scaffold are very important in tissue engineering. Of most concern high‐density seeding, nutrient oxygen supply, affinity. In the present study, poly( L ‐lactic acid) ‐lactic‐ co ‐glycolic (70/30) scaffolds with different pore structures were fabricated. An improved method based on Archimedes' Principle for measuring porosity scaffolds, using density bottle, was developed. Anhydrous ammonia plasma treatment used to modify properties improve affinity scaffolds. results show that hydrophilicity energy improved. polar N‐containing groups positive charged also incorporated into sample surface. A low‐temperature maintain plasma‐modified effectively. It would do help further application technique. Cell culture showed pores smaller than 160 μm suitable human skin fibroblast growth. seeding efficiency maintained at above 99%, which is better achieved common prewetting by ethanol. plasma‐treatment helped resolve problem loss during negative effects ethanol trace avoided. suggest anhydrous enhances Mass transport issues have been considered. © 2002 Wiley Periodicals, Inc. J Biomed Mater Res 62: 438–446,