Calcyclin binding protein promotes DNA synthesis and differentiation in rat neonatal cardiomyocytes

• DOI: 10.1002/jcb.20710
• Publication Date: 2006-01-26T21:20:44Z
• Authors: Ka‐Wing Au; Cecy Y.C. Kou; Anthony Y.H. Woo; Stephen S.C. Chim; Kwok‐Pui Fung; Christopher H.K. ...; Mary M.Y. Waye; Stephen K.W. Tsui
• Keywords: Suppression subtractive hybridization; Hypoxia

Abstract During cardiac muscle development, most cardiomyocytes permanently withdraw from the cell cycle. Previously, by suppressive subtractive hybridization, we identified calcyclin‐binding protein/Siah‐interacting protein (CacyBP/SIP) as one of candidates being upregulated in hyperplastic to hypertrophic switch, suggesting an important role CacyBP/SIP development. To show importance during myoblast differentiation, report here that is developmentally regulated postnatal rat hearts. The overexpression promotes differentiation and DNA synthesis H9C2 cells primary cardiomyocytes, well downregulates expression β‐catenin. Besides, formation myotubes multinucleation upon differentiation. investigate cardioprotective a hypoxia/reoxygenation model was employed. We found myocardial infarction (MI) hypoxia/reoxygenation. As conclusion, may play cardiomyogenic possibly protection injury. J. Cell. Biochem. 98: 555–566, 2006. © 2006 Wiley‐Liss, Inc.