Raf kinase inhibitor protein correlates with sensitivity of nasopharyngeal carcinoma to radiotherapy

• DOI: 10.1002/jcb.22611
• Publication Date: 2010-04-21T14:04:22Z
• Authors: Lin Ruan; Guo‐Liang Wang; Hong Yi; Yan Chen; Can‐E. Tang; Peng‐Fei Zhang; Mao‐Yu Li; Cui Li; Fang Peng; Jian‐Ling Li; Zhu‐Chu Chen; Zhi‐Qiang Xiao
• Keywords: Radioresistance; Radiosensitivity

Abstract Raf kinase inhibitory protein (RKIP) is a metastasis suppressor whose expression reduced in nasopharyngeal carcinoma (NPC) tissues and absent NPC metastases. To investigate the effect of RKIP on radiosensitivity NPC, high metastatic 5‐8F with low non‐metastatic 6‐10B were stably transfected plasmids that expressed sense antisense cDNA. Overexpression sensitized cells to radiation‐induced cell death, G 2 ‐M cycle arrest apoptosis. In contrast, downexpression protected from addition, altered through MEK ERK phosphorylation changes Raf‐1/MEK/ERK signaling pathway. We further investigated patients its association patients' survival after radiotherapy. Downexpression was significantly correlated advanced clinical stage, lymph node radioresistance. Furthermore, curves showed had poor prognosis induced relapse. Multivariate analysis confirmed an independent prognostic indicator. The data suggested potential biomarker for dysregulation might play important role radioresistance NPC. J. Cell. Biochem. 110: 975–984, 2010. © 2010 Wiley‐Liss, Inc.