Human umbilical cord Wharton's jelly stem cells and its conditioned medium support hematopoietic stem cell expansion ex vivo

• DOI: 10.1002/jcb.23395
• Publication Date: 2011-10-04T16:37:24Z
• Authors: C.Y. Fong; K. Gauthaman; S. Cheyyatraivendran; H.D. Lin; A. Biswas; A. Bongso
• Keywords: Wharton's jelly; Ex vivo; Cord lining; Cord blood

Bone marrow mesenchymal stromal cells (BMMSCs) have been used as feeder support for the ex vivo expansion of hematopoietic stem (HSCs) but limitations painful harvest, morbidity, and risk infection to patient. This prompted us explore use human umbilical cord Wharton's jelly MSCs (hWJSCs) its conditioned medium (hWJSC-CM) HSCs in allogeneic autologous settings because hWJSCs can be harvested abundance painlessly, are proliferative, hypoimmunogenic, secrete a variety unique proteins. In presence hWJSC-CM, put out pseudopodia-like outgrowths became highly motile. Time lapse imaging showed that helped them migrate towards attach upper surfaces undergo proliferation. After 9 days culture MTT, Trypan blue assays significant increases HSC numbers, FACS analysis generated significantly greater numbers CD34(+) compared controls. hWJSC-CM produced highest number colonies (CFU assay) all six classifications colony morphology typical hematopoiesis were observed. Proteomic levels interleukins (IL-1a, IL-6, IL-7, IL-8), SCF, HGF, ICAM-1 controls suggesting they may involved multiplication. We propose blood banks freeze (UCB) from same at time patient future cell-based therapies.