Measuring microenvironment mechanical stress of rat liver during diethylnitrosamine induced hepatocarcinogenesis by atomic force microscope

• DOI: 10.1002/jemt.20716
• Publication Date: 2009-04-07T17:48:49Z
• Authors: Zhao Gang; Qin Qi; Cui Jing; Chunyou Wang
• Keywords: Liver tissue

Abstract We developed a highly sensitive method to detect liver tissue stiffness with atomic force microscopy (AFM), and investigated the physical features of hepatocarcinogenesis. Wistar rats received weekly intraperitoneal injections diethylnitrosamine (DEN) or saline (control) followed by 2‐week wash‐out period. Liver samples were harvested at 10, 14, 18 weeks for pathological examination stress detection. Previously normal tissues fibrosis carcinoma after DEN administration. Although elastic modulus ( E ) values (saline; 0.18 ± 0.04 MPa), fibrotic (8 DEN; 0.25 0.06 MPa) cirrhotic (12 0.39 significantly different, there was no significant difference between hepatic cell (16 0.42 0.07 tissues. Thus, quantitatively increases during hepatocarcinogenesis, AFM can be used sensitively precisely microscopic level. Microsc. Res. Tech. 2009. © 2009 Wiley‐Liss, Inc.