Comparison of manual and automatic processing of biological samples for electron microscopy

• DOI: 10.1002/jemt.22832
• Publication Date: 2017-05-30T10:38:37Z
• Authors: Rui Yang; Shuai Wang; Jianli Wang; Xi Luo; Wenchao Zhao; Qing Zhang; Zhangjie Nan; Liu Yang; Shaohui Wang
• Keywords: 03 medical and health sciences; 0302 clinical medicine; Microscopy, Electron, Transmission; Staining and Labeling; Microscopy, Electron, Scanning; Specimen Handling

AbstractThe ultrastructure of a sample can be observed by electron microscopy (EM), which has become an indispensable research tool in morphological studies. However, EM sample preparation techniques are complicated and time‐consuming, with a high labor cost. The current study was conducted to compare the conventional manual and automated methods for sample processing and post‐staining for electron microscopy. Automated sample processing reduces OsO4 contamination, improves the efficiency of sample preparation and is easy to use. Therefore, the results of their study provide a practical and feasible method for the preparation of biological samples for electron microscopy.